pokeweed antiviral protein meaning in Chinese
美洲商陆抗簿蛋白
Examples
- Someone tried this way but the yield is low . francis expressed truncated mutant pokeweed antiviral protein gene in pichia pastoris
目前已经有人在这方面做出了尝试，但蛋白表达产量还不高。 - Many studies revealed that pokeweed antiviral protein ( pap ) can control plant virus effectively
许多研究发现美洲商陆抗病毒蛋白( pokeweedantiviralprotein ， pap )对植物病毒具有良好的控制效果。 - To find out the difference between mut + and muts recombinants we compared their expression of pokeweed antiviral protein in the same conditions
在相同的培养条件下比较了mut ~ +和mut ~ s重组菌株表达pap的异同。 - Linearized the expression vector ppic9k - p including the truncated mutant pokeweed antiviral protein ( pap ) gene by restriction endonuclease sal i and transformed it electrically into pichia pastoris gs115 . mut + recombinants were selected by pcr and high yield mut + recombinant was picked out by double film immunoblotting
本研究将含有n末端信号肽和c末端毒性区缺失的pap基因的表达载体ppic9k - p用sali酶切线性化后，通过电击转化整合p . pastorisgs115菌株细胞中。 - The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector . the sequencing results showed that pap gene had 99 . 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ) . the iptg - inducible expression vector containing the pap gene was constructed and transferred into e . coli bl21 ( de3 ) - plyss
将缺失型pap基因克隆到植物表达载体pbi121中，通过液氮冷冻法将重组质粒转入农杆菌lba4404细胞中，然后采用叶盘法，在该农杆菌的介导下将pap基因导入普通烟草中，经过卡那霉素抗性筛选，最后获得了转pap基因的工程烟草植株，摩擦接种烟草花叶病毒( tmv ) ，与非转基因烟草相比，能够推迟症状表现达2月之久，说明pap基因能够在其它植物体内产生有活性的高抗病毒的蛋白质。